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Image Search Results
Journal: Data in Brief
Article Title: Polymerase chain reaction-based gene removal from plasmids
doi: 10.1016/j.dib.2015.04.024
Figure Lengend Snippet: Complete DpnI digestion can be achieved in Phusion master mix. (A)?The graphic representation of pCRII-U85 plasmid marked with four segments and 28 DpnI restriction sites. Purple: u85 , cyan: f1 , green: kanR , and yellow: ampR . The expected DpnI digestion pattern is shown on the right. Both the graphic representation and the digestion pattern were generated by a software named (A Plasmid Editor (APE) developed by M. Wayne Davis), (B)?DpnI digestion of the pCRII-U85 plasmid. A total amount of 400 ng plasmid was used in each reactions. All reactions were performed at 37 °C. Lane 1: DNA ladder, Lane 2: undigested DNA, Lane 3: 30 min digestion in the fast-digestion buffer from the vendor, Lane 4: 30 min digestion in 1× Pfusion Master Mix, Lane 5: 30 min digestion in water, Lane 6: 30 min digestion in 2× Pfusion Master Mix, Lane 6: 60 min digestion in 2× Pfusion Master Mix. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)
Article Snippet:
Techniques: Plasmid Preparation, Generated, Software